Okay, I see what you mean now. What you're trying to do isn't very efficient though, this is how I'd do it.
1. Add and embed a resource of all the images to your solution:
Project > add existing item > select the images
click on the images in your solution explorer in VS, then go to the properties window and change "build action" to "embed resource"
2. Add an assembly handler to your project:
Dim asm As System.Reflection.Assembly = System.Reflection.Assembly.GetExecutingAssembly()
3. Create an image stream that will use the resource by name.
Dim ResourceName As String = "solutionName.ImageName" 'an example of the string would be "cardgame.C7.png"
Dim str As System.IO.Stream = asm.GetManifestResourceStream(ResourceName)
Dim img As Image = Image.FromStream(str)
PictureBox2.Image = img
Then just change your random code to generate the card letter and number and add that between the solution and file format.
Hopefully that helps.

"I am a game making juggalo so ha"